論文 - 詳細
| RRC ID | 56467 |
|---|---|
| 著者 | Pröschold T, Harris EH, Coleman AW. |
| タイトル | Portrait of a species: Chlamydomonas reinhardtii. |
| ジャーナル | Genetics |
| Abstract |
Chlamydomonas reinhardtii, the first alga subject to a genome project, has been the object of numerous morphological, physiological, and genetic studies. The organism has two genetically determined mating types (plus and minus) and all stages of the simple life cycle can be evoked in culture. In the nearly 60 years since the first standard laboratory strains were isolated, numerous crosses and exchanges among laboratories have led to some confusion concerning strain genealogy. Here we use analyses of the nuclear internal transcribed spacer regions and other genetic traits to resolve these issues, correctly identify strains currently available, and analyze phylogenetic relationships with all other available similar chlamydomonad types. The presence of a 10-bp indel in ITS2 in some but not all copies of the nuclear ribosomal cistrons of an individual organism, and the changing ratios of these in crosses, provide a tool to investigate mechanisms of concerted evolution. The standard C. reinhardtii strains, plus C. smithii +, plus the new eastern North American C. reinhardtii isolates, comprise one morphological species, one biological species of high sexual intercompatibility, and essentially identical ITS sequences (except the tip of helix I of ITS2). However, variant RFLP patterns characterize strains from each geographic site. |
| 巻・号 | 170(4) |
| ページ | 1601-10 |
| 公開日 | 2005-8-1 |
| DOI | 10.1534/genetics.105.044503 |
| PII | genetics.105.044503 |
| PMID | 15956662 |
| PMC | PMC1449772 |
| MeSH | Animals Base Sequence Biological Evolution Cell Nucleus / chemistry Cell Nucleus / genetics Chlamydomonas reinhardtii / classification Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / isolation & purification Chlamydomonas reinhardtii / physiology Chromosome Mapping Chromosomes Crosses, Genetic DNA, Algal DNA, Intergenic Genes Genetic Variation Genome Geography Molecular Sequence Data Nucleic Acid Conformation Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic |
| IF | 3.564 |
| 引用数 | 98 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 76 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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