RRC ID 56467
著者 Pröschold T, Harris EH, Coleman AW.
タイトル Portrait of a species: Chlamydomonas reinhardtii.
ジャーナル Genetics
Abstract Chlamydomonas reinhardtii, the first alga subject to a genome project, has been the object of numerous morphological, physiological, and genetic studies. The organism has two genetically determined mating types (plus and minus) and all stages of the simple life cycle can be evoked in culture. In the nearly 60 years since the first standard laboratory strains were isolated, numerous crosses and exchanges among laboratories have led to some confusion concerning strain genealogy. Here we use analyses of the nuclear internal transcribed spacer regions and other genetic traits to resolve these issues, correctly identify strains currently available, and analyze phylogenetic relationships with all other available similar chlamydomonad types. The presence of a 10-bp indel in ITS2 in some but not all copies of the nuclear ribosomal cistrons of an individual organism, and the changing ratios of these in crosses, provide a tool to investigate mechanisms of concerted evolution. The standard C. reinhardtii strains, plus C. smithii +, plus the new eastern North American C. reinhardtii isolates, comprise one morphological species, one biological species of high sexual intercompatibility, and essentially identical ITS sequences (except the tip of helix I of ITS2). However, variant RFLP patterns characterize strains from each geographic site.
巻・号 170(4)
ページ 1601-10
公開日 2005-8-1
DOI 10.1534/genetics.105.044503
PII genetics.105.044503
PMID 15956662
PMC PMC1449772
MeSH Animals Base Sequence Biological Evolution Cell Nucleus / chemistry Cell Nucleus / genetics Chlamydomonas reinhardtii / classification Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / isolation & purification Chlamydomonas reinhardtii / physiology Chromosome Mapping Chromosomes Crosses, Genetic DNA, Algal DNA, Intergenic Genes Genetic Variation Genome Geography Molecular Sequence Data Nucleic Acid Conformation Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic
IF 3.564
引用数 98
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