| RRC ID |
564
|
| 著者 |
Maeda T, Lee JM, Miyagawa Y, Koga K, Kawaguchi Y, Kusakabe T.
|
| タイトル |
Cloning and characterization of a ribonuclease L inhibitor from the silkworm, Bombyx mori.
|
| ジャーナル |
DNA Seq
|
| Abstract |
The ribonuclease L (RNase L) pathway plays an important role in the response of cells to double-stranded RNA (dsRNA) during the events such as virus infection. Ribonuclease L inhibitor (RLI) belonging to the ABC transporter family is known as a regulator of the RNase L pathway. The homologs of RLI were reported in many organisms including the fruit fly and mosquito, but their functions in insects and arthropods have not been elucidated to date. In the present study, we cloned a cDNA of a silkworm RLI homolog, termed BmRLI, and its nucleotide sequence was determined. RT-PCR analysis revealed that the expression of BmRLI mRNA was marked in the testis, ovary and fat body. From the cDNA, recombinant protein with an apparent molecular mass of 69 kDa was expressed in Escherichia coli and cultured insect cells. Although no obvious effect of up-regulation of the BmRLI expression on RNAi was observed, its down-regulation slightly reduced RNAi efficiency.
|
| 巻・号 |
16(1)
|
| ページ |
21-7
|
| 公開日 |
2005-2-1
|
| DOI |
10.1080/10425170400028871
|
| PII |
XXM7236763P64P74
|
| PMID |
16040343
|
| MeSH |
ATP-Binding Cassette Transporters / genetics*
Amino Acid Sequence
Animals
Base Sequence
Bombyx / genetics*
Chaperonins / genetics*
Cloning, Molecular
DNA, Complementary / genetics
Endoribonucleases / antagonists & inhibitors*
Fat Body / metabolism
Female
Gene Expression
Genes, Insect
Insect Proteins / genetics*
Male
Molecular Sequence Data
Ovary / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Testis / metabolism
Tissue Distribution
|
| 引用数 |
5
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| リソース情報 |
| カイコ |
NA |
