RRC ID 5650
著者 Takemori N, Yamamoto MT.
タイトル Proteome mapping of the Drosophila melanogaster male reproductive system.
ジャーナル Proteomics
Abstract The fruit fly Drosophila melanogaster is an excellent model organism for studying insect reproductive biology. Although the gene expression profiles of both male and female reproductive organs have been studied in detail, their proteomic profiles and functional characteristics largely remained to be clarified. In this study, we conducted proteome mapping of the male internal reproductive organs using 2-DE. We identified a total of 440 protein components from gels of the male reproductive organs (testis, seminal vesicle, accessory gland, ejaculatory duct, and ejaculatory bulb). A number of proteins associated with odorant/pheromone-binding, lipid metabolism, proteolysis, and antioxidation were expressed tissue specifically in the male reproductive system. Based on our proteomic data set, we constructed reference proteome maps of the reproductive organs, which will provide valuable information toward a comprehensive understanding of Drosophila reproduction.
巻・号 9(9)
ページ 2484-93
公開日 2009-5-1
DOI 10.1002/pmic.200800795
PMID 19343724
MeSH Animals Drosophila melanogaster / physiology* Electrophoresis, Gel, Two-Dimensional Genitalia, Male / anatomy & histology Genitalia, Male / chemistry Genitalia, Male / metabolism Insect Proteins / analysis* Insect Proteins / metabolism Male Models, Biological Peptide Mapping* Proteomics Seminal Plasma Proteins / analysis* Seminal Plasma Proteins / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 3.254
引用数 69
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
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