論文 - 詳細
| RRC ID | 56504 |
|---|---|
| 著者 | Hiramatsu K, Ideguchi T, Yonamine Y, Lee S, Luo Y, Hashimoto K, Ito T, Hase M, Park JW, Kasai Y, Sakuma S, Hayakawa T, Arai F, Hoshino Y, Goda K. |
| タイトル | High-throughput label-free molecular fingerprinting flow cytometry. |
| ジャーナル | Sci Adv |
| Abstract |
Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm-1) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris. |
| 巻・号 | 5(1) |
| ページ | eaau0241 |
| 公開日 | 2019-1-1 |
| DOI | 10.1126/sciadv.aau0241 |
| PII | aau0241 |
| PMID | 30746443 |
| PMC | PMC6357763 |
| MeSH | Carbon Dioxide / metabolism Carbon Isotopes / metabolism Chlorophyceae / metabolism Flow Cytometry / instrumentation Flow Cytometry / methods* Fourier Analysis High-Throughput Screening Assays / instrumentation High-Throughput Screening Assays / methods Lab-On-A-Chip Devices Photosynthesis Reproducibility of Results Single-Cell Analysis / instrumentation Single-Cell Analysis / methods Spectrum Analysis, Raman / instrumentation Spectrum Analysis, Raman / methods* Vibration Xanthophylls / metabolism |
| IF | 13.117 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 72 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-48 NIES-4141 NIES-144 |