RRC ID 56504
著者 Hiramatsu K, Ideguchi T, Yonamine Y, Lee S, Luo Y, Hashimoto K, Ito T, Hase M, Park JW, Kasai Y, Sakuma S, Hayakawa T, Arai F, Hoshino Y, Goda K.
タイトル High-throughput label-free molecular fingerprinting flow cytometry.
ジャーナル Sci Adv
Abstract Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm-1) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.
巻・号 5(1)
ページ eaau0241
公開日 2019-1-1
DOI 10.1126/sciadv.aau0241
PII aau0241
PMID 30746443
PMC PMC6357763
MeSH Carbon Dioxide / metabolism Carbon Isotopes / metabolism Chlorophyceae / metabolism Flow Cytometry / instrumentation Flow Cytometry / methods* Fourier Analysis High-Throughput Screening Assays / instrumentation High-Throughput Screening Assays / methods Lab-On-A-Chip Devices Photosynthesis Reproducibility of Results Single-Cell Analysis / instrumentation Single-Cell Analysis / methods Spectrum Analysis, Raman / instrumentation Spectrum Analysis, Raman / methods* Vibration Xanthophylls / metabolism
IF 13.117
引用数 24
リソース情報
藻類 NIES-48 NIES-4141 NIES-144