| RRC ID |
56602
|
| 著者 |
Tan G, Li J, Yang Q, Wu A, Qu DY, Wang Y, Ye L, Bao J, Shao Y.
|
| タイトル |
Air pollutant particulate matter 2.5 induces dry eye syndrome in mice.
|
| ジャーナル |
Sci Rep
|
| Abstract |
In this study, we explored the effects of particulate matter 2.5 (PM2.5) eye drops on the ocular surface structure and tear function in mice and established a novel animal model for dry eye research. We found that, following treatment with PM2.5, the tear volume and, the tear film break-up time showed statistical differences at each time point (P < 0.05). The FL score of the PM2.5-treated group was higher than that of others (P < 0.05). The average number of corneal epithelial layer cells in groups A and B was significantly lower than that in group C (P < 0.05). Scanning electron microscopy and transmission electron microscopy revealed that the number of corneal epithelial microvilli and corneal desmosomes was drastically reduced in group C. PM2.5 induced apoptosis in the corneal superficial and basal epithelium and led to abnormal differentiation and proliferation of the ocular surface with higher expression levels of Ki67 and a reduced number of goblet cells in the conjunctival fornix in group C. PM2.5 significantly increased the levels of TNF-α, NF-κB p65 (phospho S536), and NF-κB in the cornea. Thus, the topical administration of PM2.5 in mice induces ocular surface changes that are similar to those of dry eye in humans, representing a novel model of dry eye.
|
| 巻・号 |
8(1)
|
| ページ |
17828
|
| 公開日 |
2018-12-13
|
| DOI |
10.1038/s41598-018-36181-x
|
| PII |
10.1038/s41598-018-36181-x
|
| PMID |
30546125
|
| PMC |
PMC6292905
|
| MeSH |
Air Pollutants / toxicity*
Animals
Cell Line, Transformed
Cell Proliferation / drug effects*
Disease Models, Animal
Dry Eye Syndromes* / chemically induced
Dry Eye Syndromes* / metabolism
Dry Eye Syndromes* / pathology
Epithelium, Corneal* / metabolism
Epithelium, Corneal* / ultrastructure
Eye Proteins / biosynthesis*
Gene Expression Regulation / drug effects*
Humans
Mice
Mice, Inbred BALB C
Particulate Matter / toxicity*
|
| IF |
4.011
|
| 引用数 |
18
|
| リソース情報 |
| ヒト・動物細胞 |
HCE-T(RCB2280) |
