論文 - 詳細
| RRC ID | 56607 |
|---|---|
| 著者 | Roppongi M, Izumisawa M, Terasaki K, Muraki Y, Shozushima M. |
| タイトル | 18F-FDG and 11C-choline uptake in proliferating tumor cells is dependent on the cell cycle in vitro. |
| ジャーナル | Ann Nucl Med |
| Abstract |
OBJECTIVE:Among different PET tracers, 18F-fludeoxyglucose (FDG) and 11C-choline are known to have a high tumor uptake correlated with a high mitotic index of tumor cells. Thus, the uptake of 18F-FDG and 11C-choline may be dependent on the cell cycle. In the present study, we examined the uptake of 18F-FDG and 11C-choline in cancer cell lines by cell cycle synchronization to clarify the biological properties of cancer cells with respect to each tracer. METHODS:HeLa S3 Cells were synchronized by the double thymidine (TdR) block methods. 18F-FDG and 11C-choline were administered to synchronized cells, and the radioactivity per cell was measured to compare the cellular uptake of the tracers during S, G2/M, and G1 phases. Flow cytometry (FCM) was performed to measure the proportion of cells in G1, S, and G2/M phases. Furthermore, the levels of glucose transporter 1 (GLUT1) and choline transporter-like protein 1 (CTL1) in the cell were evaluated by FCM. RESULTS:The uptake of 18F-FDG was the highest in S to G2/M phases, and markedly decreased in G1 phase. The uptake of 11C-choline reached its peak in G2/M, and decreased in G1 phase. The level of GLUT1 expression was similar to that of 18F-FDG uptake during the cell cycle, and the level of CTL1 expression was similar to that of 11C-choline uptake throughout the cell cycle. CONCLUSIONS:In this in vitro study, we demonstrated that 18F-FDG and 11C-choline had the highest uptake in S to G2/M phases and in G2/M phase, respectively, with a rapid decrease in G1 phase. These findings suggest that 18F-FDG and 11C-choline have a high accumulation in tumor cells with a high mitotic index. Furthermore, our study suggests that the expression of GLUT1 and CTL1 has cell cycle dependence, and the changes of 18F-FDG and 11C-choline accumulation seem to be caused by the above properties of these transporters. |
| 巻・号 | 33(4) |
| ページ | 237-243 |
| 公開日 | 2019-4-1 |
| DOI | 10.1007/s12149-018-01325-6 |
| PII | 10.1007/s12149-018-01325-6 |
| PMID | 30588580 |
| PMC | PMC6450840 |
| MeSH | Antigens, CD / metabolism Biological Transport Carbon Radioisotopes* Cell Cycle* Cell Proliferation Choline / metabolism* Fluorodeoxyglucose F18 / metabolism* Gene Expression Regulation, Neoplastic Glucose Transporter Type 1 / metabolism HeLa Cells Humans Kinetics Organic Cation Transport Proteins / metabolism Positron-Emission Tomography |
| IF | 1.648 |
| 引用数 | 2 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa.S3(RCB0191) |