RRC ID 56624
Author Kuroda C, Ueda K, Haniu H, Ishida H, Okano S, Takizawa T, Sobajima A, Kamanaka T, Yoshida K, Okamoto M, Tsukahara T, Matsuda Y, Aoki K, Kato H, Saito N.
Title Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells.
Journal Int J Nanomedicine
Abstract Introduction:Carbon nanotubes (CNTs) have various shapes, including needle-like shapes and curled shapes, and the cytotoxicity and carcinogenicity of CNTs differ depending on their shapes and surface modifications. However, the biological responses induced by CNTs and related mechanisms according to the dispersion state of CNTs have not been extensively studied.
Materials and methods:We prepared multiwalled CNTs (MWCNTs) showing different dispersions and evaluated these MWCNTs in RAW264 cells to determine cytotoxicity, cellular uptake, and immune responses. Furthermore, RAW264 cells were also used to compare the cellular uptake and cytotoxicity of fibrous MWCNTs and spherical carbon nanohorns (CNHs) exhibiting the same degree of dispersion.
Results:Our analysis showed that the cellular uptake, localization, and inflammatory responses of MWCNTs differed depending on the dispersion state. Moreover, there were differences in uptake between MWCNTs and CNHs, even showing the same degree of dispersion. These findings suggested that receptors related to cytotoxicity and immune responses differed depending on the aggregated state of MWCNTs and surface modification with a dispersant. Furthermore, our results suggested that the receptors recognized by the cells differed depending on the particle shape.
Conclusion:Therefore, to apply MWCNTs as a biomaterial, it is important to determine the carcinogenicity and toxicity of the CNTs and to examine different biological responses induced by varying shapes, dispersion states, and surface modifications of particles.
Volume 13
Pages 6079-6088
Published 2018-10-5
DOI 10.2147/IJN.S172493
PII ijn-13-6079
PMID 30323595
PMC PMC6179726
MeSH Animals Cell Survival Cytokines / metabolism Fluorescent Dyes / metabolism Inflammation Mediators / metabolism Macrophages / cytology* Macrophages / metabolism Macrophages / ultrastructure Mice Nanotubes, Carbon / chemistry* Particle Size RAW 264.7 Cells Static Electricity
IF 4.471
Times Cited 5
Human and Animal Cells RAW 264(RCB0535)