RRC ID 56635
著者 Sumi C, Hirose N, Yanoshita M, Takano M, Nishiyama S, Okamoto Y, Asakawa Y, Tanimoto K.
タイトル Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress.
ジャーナル Mediators Inflamm
Abstract Background:Excessive mechanical stress causes inflammation and destruction of cartilage and is considered one of the cause of osteoarthritis (OA). Expression of semaphorin 3A (Sema3A), which is an axon guidance molecule, has been confirmed in chondrocytes. However, there are few reports about Sema3A in chondrocytes, and the effects of Sema3A on inflammation in the cartilage are poorly understood. The aim of this study was to examine the role of Sema3A in inflammation caused by high magnitude cyclic tensile strain (CTS).
Methods:Expression of Sema3A and its receptors neuropilin-1 (NRP-1) and plexin-A1 (PLXA1) in ATDC5 cells was examined by Western blot analysis. ATDC5 cells were subjected to CTS of 0.5 Hz, 10% elongation with added Sema3A for 3 h. Gene expression of IL-1β, TNF-ɑ, COX-2, MMP-3, and MMP-13 was examined by qPCR analysis. Furthermore, the phosphorylation of AKT, ERK, and NF-κB was detected by Western blot analysis.
Results:Added Sema3A inhibited the gene expression of inflammatory cytokines upregulated by CTS in a dose-dependent manner. Addition of Sema3A suppressed the activation of AKT, ERK, and NF-κB in a dose-dependent manner.
Conclusions:Sema3A reduces the gene expression of inflammatory cytokines by downregulating the activation of AKT, ERK, and NF-κB pathways in ATDC5 cells under CTS.
巻・号 2018
ページ 5703651
公開日 2018-4-8
DOI 10.1155/2018/5703651
PMID 29849491
PMC PMC5911320
MeSH Animals Blotting, Western Cell Line Chondrocytes / metabolism* Cyclooxygenase 2 / genetics Cyclooxygenase 2 / metabolism Inflammation / genetics Inflammation / metabolism* Interleukin-1beta / metabolism Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 13 / metabolism Matrix Metalloproteinase 3 / genetics Matrix Metalloproteinase 3 / metabolism Mice Neuropilin-1 / genetics Neuropilin-1 / metabolism Semaphorin-3A / genetics Semaphorin-3A / metabolism* Stress, Mechanical* Tumor Necrosis Factor-alpha / metabolism
IF 3.545
引用数 10
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)