Reference - Detail
| RRC ID | 56658 |
|---|---|
| Author | Carrasco-Rando M, Prieto-Sánchez S, Culi J, Tutor AS, Ruiz-Gómez M. |
| Title | A specific isoform of Pyd/ZO-1 mediates junctional remodeling and formation of slit diaphragms. |
| Journal | J Cell Biol |
| Abstract |
The podocyte slit diaphragm (SD), responsible for blood filtration in vertebrates, is a major target of injury in chronic kidney disease. The damage includes severe morphological changes with destabilization of SDs and their replacement by junctional complexes between abnormally broadened foot processes. In Drosophila melanogaster, SDs are present in nephrocytes, which filter the fly's hemolymph. Here, we show that a specific isoform of Polychaetoid/ZO-1, Pyd-P, is essential for Drosophila SDs, since, in pyd mutants devoid of Pyd-P, SDs do not form and the SD component Dumbfounded accumulates at ectopic septate-like junctions between abnormally aggregated nephrocytes. Reintroduction of Pyd-P leads to junctional remodeling and their progressive normalization toward SDs. This transition requires the coiled-coil domain of Pyd-P and implies formation of nonclathrin vesicles containing SD components and their trafficking to the nephrocyte external membrane, where SDs assemble. Analyses in zebrafish suggest a conserved role for Tjp1a/ZO-1 in promoting junctional remodeling in podocytes. |
| Volume | 218(7) |
| Pages | 2294-2308 |
| Published | 2019-7-1 |
| DOI | 10.1083/jcb.201810171 |
| PII | jcb.201810171 |
| PMID | 31171632 |
| PMC | PMC6605796 |
| MeSH | Animals Clathrin / genetics Diaphragm / growth & development* Drosophila Proteins / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Humans Intercellular Junctions / genetics* Kidney Glomerulus / growth & development Kidney Glomerulus / metabolism Mutant Proteins / genetics Podocytes / metabolism* Protein Isoforms / genetics Tight Junction Proteins / genetics* Zebrafish / genetics |
| IF | 8.891 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 30 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 9763R-2 |