RRC ID 56693
Author Song J, Ugai H, Kanazawa I, Sun K, Yokoyama KK.
Title Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements.
Journal J Biol Chem
Abstract The transcription factors Sp1 and MAZ (Myc-associated zinc finger protein) contain several zinc finger motifs, and each functions as both a positive and a negative regulator of gene expression. In this study, we characterized the extremely GC-rich promoter of the human gene for MAZ, which is known as a housekeeping gene. Unique symmetrical motifs in the promoter region (nucleotides -383 to -334) were essential for the expression of the gene for MAZ, whereas an upstream silencer element (nucleotides -784 to -612) was found to act in a position-dependent but orientation-independent manner. Sp1 and MAZ bound to the same cis-elements in the GC-rich promoter, apparently sharing DNA-binding sites. The relative extent of binding of Sp1 and MAZ to these cis-elements corresponded to the extent of negative regulation of the expression of the gene for MAZ in various lines of cells. Furthermore, novel repressive domains in both Sp1 (amino acids 622-788) and MAZ (amino acids 127-292) were identified. Suppression by Sp1 and suppression by MAZ were independent phenomena; histone deacetylases were involved in the autorepression by MAZ itself, whereas DNA methyltransferase 1 was associated with suppression by Sp1. Our results indicate that both deacetylation and methylation might be involved in the regulation of expression of a single gene via the actions of different zinc finger proteins that bind to the same cis-elements.
Volume 276(23)
Pages 19897-904
Published 2001-6-8
DOI 10.1074/jbc.M010658200
PII S0021-9258(19)40420-1
PMID 11259406
MeSH Animals Base Sequence Cell Line DNA DNA-Binding Proteins Gene Expression Regulation / physiology* Histone Deacetylases / metabolism Humans Molecular Sequence Data Precipitin Tests Promoter Regions, Genetic Proto-Oncogene Proteins c-myc / physiology* Sp1 Transcription Factor / physiology* Transcription Factors / genetics* Transcription Factors / physiology* Zinc Fingers
IF 4.106
Times Cited 46
DNA material pCMV-SP1 (RDB04267) pCMV-SP1 delta 1-137 (RDB04268) pCMV-SP1 delta 1-246 (RDB04269) pCMV-SP1 delta 1-436 (RDB04270) pCMV-SP1 delta 309-530 (RDB04271) pCMV-MAZ delta 1-53 (RDB04272) pCMV-MAZ delta 54-195 (RDB04273) pCMV-MAZ delta 127-292 (RDB04274)