Reference - Detail
|Author||Hara Y, Arai MA, Sakai K, Ishikawa N, Gonoi T, Yaguchi T, Ishibashi M.|
|Title||Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells.|
|Journal||J Nat Med|
A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from D-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a "co-culture" in which the genus Nocardia was cultured in the presence of an animal cell line.
|MeSH||Animals Bacterial Proteins / genetics Biosynthetic Pathways Cell Line Coculture Techniques Host-Pathogen Interactions Macrophages / microbiology Mice Nocardia / genetics Nocardia / metabolism* Nocardia Infections / metabolism Nocardia Infections / microbiology Pantothenic Acid / analogs & derivatives* Pantothenic Acid / biosynthesis Pantothenic Acid / chemistry Pantothenic Acid / isolation & purification* Phylogeny|
|Pathogenic eukaryotic microorganisms||病原放線菌：IFM 10554|