RRC ID 56863
Author Hara Y, Arai MA, Sakai K, Ishikawa N, Gonoi T, Yaguchi T, Ishibashi M.
Title Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells.
Journal J Nat Med
Abstract A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from D-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a "co-culture" in which the genus Nocardia was cultured in the presence of an animal cell line.
Volume 72(1)
Pages 280-289
Published 2018-1
DOI 10.1007/s11418-017-1161-y
PII 10.1007/s11418-017-1161-y
PMID 29209902
MeSH Animals Bacterial Proteins / genetics Biosynthetic Pathways Cell Line Coculture Techniques Host-Pathogen Interactions Macrophages / microbiology Mice Nocardia / genetics Nocardia / metabolism* Nocardia Infections / metabolism Nocardia Infections / microbiology Pantothenic Acid / analogs & derivatives* Pantothenic Acid / biosynthesis Pantothenic Acid / chemistry Pantothenic Acid / isolation & purification* Phylogeny
IF 1.966
Pathogenic eukaryotic microorganisms 病原放線菌:IFM 10554