| RRC ID |
56866
|
| Author |
Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K.
|
| Title |
The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
|
| Journal |
J Gen Appl Microbiol
|
| Abstract |
Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina. P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans. N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS). Analyses revealed the presence of neutral N-linked glycans ranging in length from Man3GlcNAc2-PA to Man9GlcNAc2-PA. The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A). The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively. In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant. The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed. This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.
|
| Volume |
65(2)
|
| Pages |
53-63
|
| Published |
2019-5-21
|
| DOI |
10.2323/jgam.2018.05.003
|
| PMID |
30305477
|
| MeSH |
Basidiomycota / chemistry
Chromatography, High Pressure Liquid
Fungal Polysaccharides / chemistry*
Fungal Polysaccharides / metabolism
Glycoside Hydrolases / metabolism
Isomerism
Malassezia / chemistry*
Mass Spectrometry
Monosaccharides / chemistry
Ustilaginales / chemistry*
|
| IF |
0.789
|
| Times Cited |
0
|
| Resource |
| Pathogenic eukaryotic microorganisms |
IFM 48685 |
