RRC ID 56929
Author Kheawfu K, Pikulkaew S, Hamamoto H, Sekimizu K, Okonogi S.
Title Influence of clove oil and eugenol on muscle contraction of silkworm (Bombyx mori).
Journal Drug Discov Ther
Abstract Clove oil is used in fish anesthesia and expected to have a mechanism via glutamic receptor. The present study explores the activities of clove oil and its major compound, eugenol, in comparison with L-glutamic acid on glutamic receptor of silkworm muscle and fish anesthesia. It was found that clove oil and eugenol had similar effects to L-glutamic acid on inhibition of silkworm muscle contraction after treated with D-glutamic acid and kainic acid. Anesthetic activity of the test samples was investigated in goldfish. The results demonstrated that L-glutamic acid at 20 and 40 mM could induce the fish to stage 3 of anesthesia that the fish exhibited total loss of equilibrium and muscle tone, whereas clove oil and eugenol at 60 ppm could induce the fish to stage 4 of anesthesia that the reflex activity of the fish was lost. These results suggest that clove oil and eugenol have similar functional activities and mechanism to L-glutamic acid on muscle contraction and fish anesthesia.
Volume 11(2)
Pages 64-69
Published 2017-5-30
DOI 10.5582/ddt.2017.01012
PMID 28458297
MeSH Anesthesia Animals Anti-Infective Agents / pharmacology* Bombyx Clove Oil / pharmacology* Eugenol / pharmacology* Glutamic Acid / pharmacology Goldfish Kainic Acid / pharmacology Muscle Contraction / drug effects* Muscle Tonus / drug effects* Postural Balance / drug effects Receptors, Glutamate Reflex / drug effects
Times Cited 1