| RRC ID |
57006
|
| 著者 |
Kitta R, Yamahama Y, Yamamoto T, Mase K, Sawada H.
|
| タイトル |
Molecular Characterization and Tissue Distribution of Mitochondrial Ca2+ -Dependent Solute Carrier Protein during Prevention of Diapause by HCl Treatment in the Silkworm, Bombyx mori.
|
| ジャーナル |
Zoolog Sci
|
| Abstract |
To clarify the molecular mechanism of prevention of entry into diapause in Bombyx mori by HCl treatment, we biochemically analyzed mitochondrial Ca2+ -dependent solute carrier protein (MCSC) in diapause eggs treated with HCl solution. Our previous studies revealed that HCl treatment causes Ca2+ to efflux from diapause eggs. Therefore, we attempted to analyze MCSC, which is known to associate with Ca2+ . The isolated cDNA of B. mori MCSC (BmMCSC) had an open reading flame (ORF) of 667 amino acid residues, and the ORF contained two EF-hand calcium-binding domains and three characteristic features of the mitochondrial solute carrier superfamily. The gene expression level of BmMCSC increased by HCl treatment. A Ca2+ binding assay indicated that recombinant BmMCSC (rBmMCSC) shows an affinity with Ca2 + . The distribution of BmMCSC was investigated with an immunohistochemical technique using antisera against BmMCSC in diapause eggs and HCl-treated diapause eggs. BmMCSC was localized in serosa cells in both eggs. These data may suggest that BmMCSC is activated by intracellular Ca2+ or efflux Ca2+ by HCl treatment, and that it plays a role in the molecular mechanisms of artificial diapause prevention or the breaking of diapause in the silkworm.
|
| 巻・号 |
35(6)
|
| ページ |
487-493
|
| 公開日 |
2018-12-4
|
| DOI |
10.2108/zs180041
|
| PMID |
30520362
|
| MeSH |
Animals
Bombyx / drug effects
Bombyx / metabolism*
Gene Expression Regulation, Developmental / drug effects
Hydrochloric Acid / pharmacology*
Insect Proteins / genetics
Insect Proteins / metabolism*
Mitochondrial Proteins / genetics
Mitochondrial Proteins / metabolism*
Tissue Distribution
|
| IF |
0.846
|
| 引用数 |
1
|
| リソース情報 |
| カイコ |
p50 |
