RRC ID 57015
Author Muhammad A, Toufeeq S, Yu HZ, Wang J, Zhang SZ, Li B, Li Z, Yang LA, Hu P, Ma Y, Xu JP.
Title Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection.
Journal J Insect Sci
Abstract Proteins p38 map kinase and ribosomal S6 kinase (S6K) as members of mitogen-activated protein kinases (MAPKs) play important roles against pathogens. In this study, Bmp38 and BmS6K were identified as differentially expressed proteins from iTRAQ database. Bmp38 and BmS6K were expressed, and recombinant proteins were purified. The bioinformatics analysis showed that both proteins have serine/threonine-protein kinases, catalytic domain (S_TKc) with 360 and 753 amino acids, respectively. The real-time quantitative polymerase chain reaction (RT-qPCR) results suggest that Bmp38 and BmS6K had high expression in the midgut and hemolymph. The comparative expression level of Bmp38 and BmS6K in BC9 was upregulated than in P50 in the midgut after Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Western bolt results showed a positive correlation between RT-qPCR and iTRAQ data for Bmp38, but BmS6K data showed partial correlation with iTRAQ. Injection of anti-Bmp38 and anti-BmS6K serum suggested that Bmp38 may be involved against BmNPV infection, whereas BmS6K may require phosphorylation modification to inhibit BmNPV infection. Taken together, our results suggest that Bmp38 and BmS6k might play an important role in innate immunity of silkworm against BmNPV.
Volume 19(1)
Published 2019-1-1
DOI 10.1093/jisesa/iey134
PII 5306023
PMID 30715437
PMC PMC6359879
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Bombyx / growth & development Bombyx / immunology Bombyx / virology Immunity, Innate / genetics Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Larva / genetics Larva / growth & development Larva / immunology Larva / virology Nucleopolyhedroviruses / physiology* Phylogeny Ribosomal Protein S6 Kinases / chemistry Ribosomal Protein S6 Kinases / genetics* Ribosomal Protein S6 Kinases / metabolism Sequence Alignment p38 Mitogen-Activated Protein Kinases / chemistry p38 Mitogen-Activated Protein Kinases / genetics* p38 Mitogen-Activated Protein Kinases / metabolism
IF 1.446
Times Cited 3
Silkworms p50 a35