論文 - 詳細
| RRC ID | 57021 |
|---|---|
| 著者 | Permana D, Minamihata K, Tatsuke T, Lee JM, Kusakabe T, Goto M, Kamiya N. |
| タイトル | Polymerization of Horseradish Peroxidase by a Laccase-Catalyzed Tyrosine Coupling Reaction. |
| ジャーナル | Biotechnol J |
| Abstract |
The polymerization of proteins can create newly active and large bio-macromolecular assemblies that exhibit unique functionalities depending on the properties of the building block proteins and the protein units in polymers. Herein, the first enzymatic polymerization of horseradish peroxidase (HRP) is reported. Recombinant HRPs fused with a tyrosine-tag (Y-tag) through a flexible linker at the N- and/or C-termini are expressed in silkworm, Bombyx mori. Trametes sp. laccase (TL) is used to activate the tyrosine of Y-tagged HRPs with molecular O2 to form a tyrosyl-free radical, which initiates the tyrosine coupling reaction between the HRP units. A covalent dityrosine linkage is also formed through a HRP-catalyzed self-crosslinking reaction in the presence of H2 O2 . The addition of H2 O2 in the self-polymerization of Y-tagged HRPs results in lower activity of the HRP polymers, whereas TL provides site-selectivity, mild reaction conditions and maintains the activity of the polymeric products. The cocrosslinking of Y-tagged HRPs and HRP-protein G (Y-HRP-pG) units catalyzed by TL shows a higher signal in enzyme-linked immunosorbent assay (ELISA) than the genetically pG-fused HRP, Y-HRP-pG, and its polymers. This new enzymatic polymerization of HRP promises to provide highly active and functionalized polymers for biomedical applications and diagnostics probes. |
| 巻・号 | 14(6) |
| ページ | e1800531 |
| 公開日 | 2019-6-1 |
| DOI | 10.1002/biot.201800531 |
| PMID | 30810287 |
| MeSH | Enzyme-Linked Immunosorbent Assay Horseradish Peroxidase / chemistry* Horseradish Peroxidase / metabolism* Laccase / metabolism* Polymerization Tyrosine / chemistry* Tyrosine / metabolism* |
| IF | 3.543 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | f38 |