論文 - 詳細
| RRC ID | 5704 |
|---|---|
| 著者 | Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M. |
| タイトル | An auxin-based degron system for the rapid depletion of proteins in nonplant cells. |
| ジャーナル | Nat Methods |
| Abstract |
Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function. |
| 巻・号 | 6(12) |
| ページ | 917-22 |
| 公開日 | 2009-12-1 |
| DOI | 10.1038/nmeth.1401 |
| PII | nmeth.1401 |
| PMID | 19915560 |
| MeSH | Animals Cell Line Chickens Green Fluorescent Proteins / metabolism Indoleacetic Acids / metabolism* Proteins / metabolism* SKP Cullin F-Box Protein Ligases / metabolism Saccharomyces cerevisiae / metabolism |
| IF | 30.822 |
| 引用数 | 573 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| 遺伝子材料 | pNHK60 (RDB08468) pMK106 (RDB08469) pMK107 (RDB08470) |
| 酵母 | BYP6739-BYP6744 BY255576-BY25603 |
| ヒト・動物細胞 | HeLa(RCB0007) COS-1(RCB0143) NIH3T3 293(RCB1637) |