RRC ID 5704
Author Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M.
Title An auxin-based degron system for the rapid depletion of proteins in nonplant cells.
Journal Nat. Methods
Abstract Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function.
Volume 6(12)
Pages 917-22
Published 2009-12
DOI 10.1038/nmeth.1401
PII nmeth.1401
PMID 19915560
MeSH Animals Cell Line Chickens Green Fluorescent Proteins / metabolism Indoleacetic Acids / metabolism* Proteins / metabolism* SKP Cullin F-Box Protein Ligases / metabolism Saccharomyces cerevisiae / metabolism
IF 26.919
Times Cited 273
WOS Category BIOCHEMICAL RESEARCH METHODS
Resource
DNA material pNHK60 (RDB08468) pMK106 (RDB08469) pMK107 (RDB08470)
Yeast BYP6739-BYP6744 BY255576-BY25603