RRC ID 57048
Author Okuzaki Y, Kidani S, Kaneoka H, Iijima S, Nishijima KI.
Title Characterization of chicken interferon-inducible transmembrane protein-10.
Journal Biosci Biotechnol Biochem
Abstract Interferon-inducible transmembrane protein (IFITM) family proteins are antivirus factors. In the present study, we examined the expression pattern of chicken IFITM10 using quantitative reverse transcription-polymerase chain reaction. In adult chickens, IFITM10 levels were markedly lower than those of IFITM3, which exhibits antivirus activity. On the other hand, IFITM10 was expressed in levels similar to those of IFITM3 in embryonic organs. Primordial germ cells in 2.5-d embryos expressed high levels of IFITM10, which gradually decreased with time. The interferon-α stimulation of embryonic fibroblast cells did not enhance the expression of IFITM10. The forced expression of IFITM10 slightly inhibited the infectivity of the VSV-G-pseudotyped lentiviral vector. Furthermore, cell fusion was inhibited by IFITM10 when HeLa cells transfected with the VSV-G expression vector were treated with low pH buffer. Although it remains unclear whether IFITM10 inhibits viral infections under physiological conditions, these results suggest that chicken IFITM10 exhibits antivirus activity.
Volume 81(5)
Pages 914-921
Published 2017-5-1
DOI 10.1080/09168451.2016.1274639
PMID 28084173
MeSH Animals Chemokine CXCL10 / genetics* Chemokine CXCL10 / metabolism* Chick Embryo Chickens / genetics Chickens / metabolism* Chickens / virology Cloning, Molecular Gene Expression Regulation, Developmental Genetic Vectors / genetics HeLa Cells Humans Interferon-alpha / pharmacology Lentivirus / genetics Membrane Glycoproteins / genetics Viral Envelope Proteins / genetics
IF 1.516
Times Cited 2
Chicken / Quail