RRC ID 57049
Author Mizushima S, Hiyama G, Shiba K, Inaba K, Dohra H, Ono T, Shimada K, Sasanami T.
Title The birth of quail chicks after intracytoplasmic sperm injection.
Journal Development
Abstract Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors - phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) - all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca(2+) rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca(2+) oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.
Volume 141(19)
Pages 3799-806
Published 2014-10
DOI 10.1242/dev.111765
PII 141/19/3799
PMID 25249465
MeSH Aconitate Hydratase / analysis Animals Calcium / metabolism Chromatography, Liquid Citrate (si)-Synthase / analysis Fertilization / physiology* Immunoblotting Male Microscopy, Fluorescence Ovum / metabolism Phosphoinositide Phospholipase C / analysis Quail / physiology* Sperm Injections, Intracytoplasmic / methods Sperm Injections, Intracytoplasmic / veterinary* Spermatozoa / chemistry* Tandem Mass Spectrometry Treatment Outcome
IF 5.763
Times Cited 18
Resource
Chicken / Quail