RRC ID 57063
Author Malolepszy A, Kelly S, Sørensen KK, James EK, Kalisch C, Bozsoki Z, Panting M, Andersen SU, Sato S, Tao K, Jensen DB, Vinther M, Jong N, Madsen LH, Umehara Y, Gysel K, Berentsen MU, Blaise M, Jensen KJ, Thygesen MB, Sandal N, Andersen KR, Radutoiu S.
Title A plant chitinase controls cortical infection thread progression and nitrogen-fixing symbiosis.
Journal Elife
Abstract Morphogens provide positional information and their concentration is key to the organized development of multicellular organisms. Nitrogen-fixing root nodules are unique organs induced by Nod factor-producing bacteria. Localized production of Nod factors establishes a developmental field within the root where plant cells are reprogrammed to form infection threads and primordia. We found that regulation of Nod factor levels by Lotus japonicus is required for the formation of nitrogen-fixing organs, determining the fate of this induced developmental program. Our analysis of plant and bacterial mutants shows that a host chitinase modulates Nod factor levels possibly in a structure-dependent manner. In Lotus, this is required for maintaining Nod factor signalling in parallel with the elongation of infection threads within the nodule cortex, while root hair infection and primordia formation are not influenced. Our study shows that infected nodules require balanced levels of Nod factors for completing their transition to functional, nitrogen-fixing organs.
Volume 7
Published 2018-10-4
DOI 10.7554/eLife.38874
PII 38874
PMID 30284535
PMC PMC6192697
MeSH Chitinases / genetics* Chitinases / metabolism Gene Expression Regulation, Plant Lipopolysaccharides / genetics Lotus / chemistry Lotus / genetics Nitrogen / metabolism Nitrogen-Fixing Bacteria / genetics* Nitrogen-Fixing Bacteria / metabolism Plant Roots / metabolism Plant Roots / microbiology Root Nodules, Plant / genetics Root Nodules, Plant / microbiology* Symbiosis / genetics*
IF 7.551
Times Cited 8
Lotus / Glycine DNA