RRC ID 57087
Author Kerstens A, Corthout N, Pavie B, Huang Z, Vernaillen F, Vande Velde G, Munck S.
Title A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples.
Journal BMC Biol
Abstract BACKGROUND:Current mesoscale 3D imaging techniques are limited to transparent or cleared samples or require the use of X-rays. This is a severe limitation for many research areas, as the 3D color surface morphology of opaque samples-for example, intact adult Drosophila, Xenopus embryos, and other non-transparent samples-cannot be assessed. We have developed "ALMOST," a novel optical method for 3D surface imaging of reflective opaque objects utilizing an optical projection tomography device in combination with oblique illumination and optical filters.
RESULTS:As well as demonstrating image formation, we provide background information and explain the reconstruction-and consequent rendering-using a standard filtered back projection algorithm and 3D software. We expanded our approach to fluorescence and multi-channel spectral imaging, validating our results with micro-computed tomography. Different biological and inorganic test samples were used to highlight the versatility of our approach. To further demonstrate the applicability of ALMOST, we explored the muscle-induced form change of the Drosophila larva, imaged adult Drosophila, dynamically visualized the closure of neural folds during neurulation of live Xenopus embryos, and showed the complementarity of our approach by comparison with transmitted light and fluorescence OPT imaging of a Xenopus tadpole.
CONCLUSION:Thus, our new modality for spectral/color, macro/mesoscopic 3D imaging can be applied to a variety of model organisms and enables the longitudinal surface dynamics during development to be revealed.
Volume 17(1)
Pages 1
Published 2019-1-7
DOI 10.1186/s12915-018-0614-4
PII 10.1186/s12915-018-0614-4
PMID 30616566
PMC PMC6323867
MeSH Animals Drosophila Imaging, Three-Dimensional / methods* Tomography, Optical / methods* Xenopus
IF 6.723
Times Cited 4
Drosophila DGRC#105666