論文 - 詳細
| RRC ID | 57093 |
|---|---|
| 著者 | Hamid R, Hajirnis N, Kushwaha S, Saleem S, Kumar V, Mishra RK. |
| タイトル | Drosophila Choline transporter non-canonically regulates pupal eclosion and NMJ integrity through a neuronal subset of mushroom body. |
| ジャーナル | Dev Biol |
| Abstract |
Insect mushroom bodies (MB) have an ensemble of synaptic connections well-studied for their role in experience-dependent learning and several higher cognitive functions. MB requires neurotransmission for an efficient flow of information across synapses with different flexibility to meet the demand of the dynamically changing environment of an insect. Neurotransmitter transporters coordinate appropriate changes for an efficient neurotransmission at the synapse. Till date, there is no transporter reported for any of the previously known neurotransmitters in the intrinsic neurons of MB. In this study, we report a highly enriched expression of Choline Transporter (ChT) in Drosophila MB. We demonstrate that knockdown of ChT in a sub-type of MB neurons called α/β core (α/βc) and ϒ neurons leads to eclosion failure, peristaltic defect in larvae, and altered NMJ phenotype. These defects were neither observed on knockdown of proteins of the cholinergic locus in α/βc and ϒ neurons nor by knockdown of ChT in cholinergic neurons. Thus, our study provides insights into non-canonical roles of ChT in MB. |
| 巻・号 | 446(1) |
| ページ | 80-93 |
| 公開日 | 2019-2-1 |
| DOI | 10.1016/j.ydbio.2018.12.006 |
| PII | S0012-1606(18)30517-7 |
| PMID | 30529058 |
| MeSH | Animals Animals, Genetically Modified Cholinergic Neurons / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Larva / genetics Larva / growth & development Larva / metabolism Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Mushroom Bodies / cytology Mushroom Bodies / growth & development Mushroom Bodies / metabolism* Neuromuscular Junction / genetics Neuromuscular Junction / growth & development Neuromuscular Junction / metabolism* Neurons / metabolism* Pupa / genetics Pupa / growth & development Pupa / metabolism RNA Interference Synaptic Transmission / genetics Synaptic Transmission / physiology |
| IF | 2.936 |
| 引用数 | 0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#105080 DGRC#103898 |