論文 - 詳細
| RRC ID | 57106 |
|---|---|
| 著者 | Huang H, Liu S, Kornberg TB. |
| タイトル | Glutamate signaling at cytoneme synapses. |
| ジャーナル | Science |
| Abstract |
We investigated the roles of components of neuronal synapses for development of the Drosophila air sac primordium (ASP). The ASP, an epithelial tube, extends specialized signaling filopodia called cytonemes that take up signals such as Dpp (Decapentaplegic, a homolog of the vertebrate bone morphogenetic protein) from the wing imaginal disc. Dpp signaling in the ASP was compromised if disc cells lacked Synaptobrevin and Synaptotagmin-1 (which function in vesicle transport at neuronal synapses), the glutamate transporter, and a voltage-gated calcium channel, or if ASP cells lacked Synaptotagmin-4 or the glutamate receptor GluRII. Transient elevations of intracellular calcium in ASP cytonemes correlate with signaling activity. Calcium transients in ASP cells depend on GluRII, are activated by l-glutamate and by stimulation of an optogenetic ion channel expressed in the wing disc, and are inhibited by EGTA and by the GluR inhibitor NASPM (1-naphthylacetyl spermine trihydrochloride). Activation of GluRII is essential but not sufficient for signaling. Cytoneme-mediated signaling is glutamatergic. |
| 巻・号 | 363(6430) |
| ページ | 948-955 |
| 公開日 | 2019-3-1 |
| DOI | 10.1126/science.aat5053 |
| PII | 363/6430/948 |
| PMID | 30819957 |
| PMC | PMC7008667 |
| MeSH | Animals Animals, Genetically Modified Calcium Channels / physiology Calcium Signaling* Drosophila Proteins / physiology* Drosophila melanogaster / physiology Glutamates / physiology* Imaginal Discs / physiology* Optical Imaging Pseudopodia / physiology R-SNARE Proteins / physiology Receptors, Ionotropic Glutamate / physiology* Sarcoplasmic Reticulum Calcium-Transporting ATPases / physiology Synapses / physiology* Synaptotagmin I / physiology Tissue Culture Techniques |
| IF | 41.063 |
| 引用数 | 18 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 6992R-1 |