RRC ID 57148
著者 Delgado MG, Oliva C, López E, Ibacache A, Galaz A, Delgado R, Barros LF, Sierralta J.
タイトル Chaski, a novel Drosophila lactate/pyruvate transporter required in glia cells for survival under nutritional stress.
ジャーナル Sci Rep
Abstract The intercellular transport of lactate is crucial for the astrocyte-to-neuron lactate shuttle (ANLS), a model of brain energetics according to which neurons are fueled by astrocytic lactate. In this study we show that the Drosophila chaski gene encodes a monocarboxylate transporter protein (MCT/SLC16A) which functions as a lactate/pyruvate transporter, as demonstrated by heterologous expression in mammalian cell culture using a genetically encoded FRET nanosensor. chaski expression is prominent in the Drosophila central nervous system and it is particularly enriched in glia over neurons. chaski mutants exhibit defects in a high energy demanding process such as synaptic transmission, as well as in locomotion and survival under nutritional stress. Remarkably, locomotion and survival under nutritional stress defects are restored by chaski expression in glia cells. Our findings are consistent with a major role for intercellular lactate shuttling in the brain metabolism of Drosophila.
巻・号 8(1)
ページ 1186
公開日 2018-1-19
DOI 10.1038/s41598-018-19595-5
PII 10.1038/s41598-018-19595-5
PMID 29352169
PMC PMC5775259
MeSH Amino Acid Sequence Animals Cell Line Cell Survival Chromosome Pairing Drosophila Humans Membrane Transport Proteins / chemistry Membrane Transport Proteins / genetics* Membrane Transport Proteins / metabolism Monocarboxylic Acid Transporters / chemistry Monocarboxylic Acid Transporters / genetics* Monocarboxylic Acid Transporters / metabolism Neuroglia / metabolism* Neurons / metabolism Stress, Physiological*
IF 4.011
引用数 7
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