論文 - 詳細
| RRC ID | 57163 |
|---|---|
| 著者 | Yao C, Wang C, Li Y, Zavortink M, Archambault V, Girton J, Johansen KM, Johansen J. |
| タイトル | Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation. |
| ジャーナル | PLoS One |
| Abstract |
In Drosophila it has recently been demonstrated that a spindle matrix in the form of a membrane-less macromolecular assembly embeds the microtubule-based spindle apparatus. In addition, two of its constituents, Megator and Chromator, were shown to function as spatial regulators of spindle checkpoint proteins. However, whether the spindle matrix plays a wider functional role in spatially regulating cell cycle progression factors was unknown. Here using a live imaging approach we provide evidence that a number of key cell cycle proteins such as Cyclin B, Polo, and Ran co-localize with the spindle matrix during mitosis. Furthermore, prevention of spindle matrix formation by injection of a function blocking antibody against the spindle matrix protein Chromator results in cell cycle arrest prior to nuclear envelope breakdown. In such embryos the spatial dynamics of Polo and Cyclin B enrichment at the nuclear rim and kinetochores is abrogated and Polo is not imported into the nucleus. This is in contrast to colchicine-arrested embryos where the wild-type dynamics of these proteins are maintained. Taken together these results suggest that spindle matrix formation may be a general requirement for the localization and proper dynamics of cell cycle factors promoting signaling events leading to cell cycle progression. |
| 巻・号 | 13(11) |
| ページ | e0208022 |
| 公開日 | 2018-11-28 |
| DOI | 10.1371/journal.pone.0208022 |
| PII | PONE-D-18-23551 |
| PMID | 30485354 |
| PMC | PMC6261614 |
| MeSH | Animals Animals, Genetically Modified Antibodies / metabolism Cell Cycle / drug effects Cell Cycle / physiology* Cell Cycle Proteins / metabolism* Colchicine / pharmacology Drosophila Proteins / metabolism* Drosophila melanogaster Embryonic Development / drug effects Embryonic Development / physiology Spindle Apparatus / metabolism* Tubulin Modulators / pharmacology |
| IF | 2.776 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#110579 DGRC#110624 DGRC#115129 |