| RRC ID |
57163
|
| 著者 |
Yao C, Wang C, Li Y, Zavortink M, Archambault V, Girton J, Johansen KM, Johansen J.
|
| タイトル |
Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation.
|
| ジャーナル |
PLoS One
|
| Abstract |
In Drosophila it has recently been demonstrated that a spindle matrix in the form of a membrane-less macromolecular assembly embeds the microtubule-based spindle apparatus. In addition, two of its constituents, Megator and Chromator, were shown to function as spatial regulators of spindle checkpoint proteins. However, whether the spindle matrix plays a wider functional role in spatially regulating cell cycle progression factors was unknown. Here using a live imaging approach we provide evidence that a number of key cell cycle proteins such as Cyclin B, Polo, and Ran co-localize with the spindle matrix during mitosis. Furthermore, prevention of spindle matrix formation by injection of a function blocking antibody against the spindle matrix protein Chromator results in cell cycle arrest prior to nuclear envelope breakdown. In such embryos the spatial dynamics of Polo and Cyclin B enrichment at the nuclear rim and kinetochores is abrogated and Polo is not imported into the nucleus. This is in contrast to colchicine-arrested embryos where the wild-type dynamics of these proteins are maintained. Taken together these results suggest that spindle matrix formation may be a general requirement for the localization and proper dynamics of cell cycle factors promoting signaling events leading to cell cycle progression.
|
| 巻・号 |
13(11)
|
| ページ |
e0208022
|
| 公開日 |
2018-11-28
|
| DOI |
10.1371/journal.pone.0208022
|
| PII |
PONE-D-18-23551
|
| PMID |
30485354
|
| PMC |
PMC6261614
|
| MeSH |
Animals
Animals, Genetically Modified
Antibodies / metabolism
Cell Cycle / drug effects
Cell Cycle / physiology*
Cell Cycle Proteins / metabolism*
Colchicine / pharmacology
Drosophila Proteins / metabolism*
Drosophila melanogaster
Embryonic Development / drug effects
Embryonic Development / physiology
Spindle Apparatus / metabolism*
Tubulin Modulators / pharmacology
|
| IF |
2.776
|
| 引用数 |
1
|
| リソース情報 |
| ショウジョウバエ |
DGRC#110579
DGRC#110624
DGRC#115129 |
