Reference - Detail
| RRC ID | 57176 |
|---|---|
| Author | Nishioka K, Wang XF, Miyazaki H, Soejima H, Hirose S. |
| Title | Mbf1 ensures Polycomb silencing by protecting E(z) mRNA from degradation by Pacman. |
| Journal | Development |
| Abstract |
Under stress conditions, the coactivator Multiprotein bridging factor 1 (Mbf1) translocates from the cytoplasm into the nucleus to induce stress-response genes. However, its role in the cytoplasm, where it is mainly located, has remained elusive. Here, we show that Drosophila Mbf1 associates with E(z) mRNA and protects it from degradation by the exoribonuclease Pacman (Pcm), thereby ensuring Polycomb silencing. In genetic studies, loss of mbf1 function enhanced a Polycomb phenotype in Polycomb group mutants, and was accompanied by a significant reduction in E(z) mRNA expression. Furthermore, a pcm mutation suppressed the Polycomb phenotype and restored the expression level of E(z) mRNA, while pcm overexpression exhibited the Polycomb phenotype in the mbf1 mutant but not in the wild-type background. In vitro, Mbf1 protected E(z) RNA from Pcm activity. Our results suggest that Mbf1 buffers fluctuations in Pcm activity to maintain an E(z) mRNA expression level sufficient for Polycomb silencing. |
| Volume | 145(5) |
| Published | 2018-3-9 |
| DOI | 10.1242/dev.162461 |
| PII | 145/5/dev162461 |
| PMID | 29523653 |
| PMC | PMC5868998 |
| MeSH | Animals Animals, Genetically Modified Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Drosophila melanogaster Exoribonucleases / genetics Exoribonucleases / metabolism* Female Male Nuclear Proteins / genetics* Nuclear Proteins / metabolism Polycomb Repressive Complex 1 / genetics* Polycomb Repressive Complex 2 / genetics* Polycomb Repressive Complex 2 / metabolism RNA Interference* RNA Stability / genetics* Trans-Activators / physiology* |
| IF | 5.763 |
| Times Cited | 4 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |