RRC ID 57180
著者 Widmer YF, Fritsch C, Jungo MM, Almeida S, Egger B, Sprecher SG.
タイトル Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit.
ジャーナル Elife
Abstract Lasting changes in gene expression are critical for the formation of long-term memories (LTMs), depending on the conserved CrebB transcriptional activator. While requirement of distinct neurons in defined circuits for different learning and memory phases have been studied in detail, only little is known regarding the gene regulatory changes that occur within these neurons. We here use the fruit fly as powerful model system to study the neural circuits of CrebB-dependent appetitive olfactory LTM. We edited the CrebB locus to create a GFP-tagged CrebB conditional knockout allele, allowing us to generate mutant, post-mitotic neurons with high spatial and temporal precision. Investigating CrebB-dependence within the mushroom body (MB) circuit we show that MB α/β and α'/β' neurons as well as MBON α3, but not in dopaminergic neurons require CrebB for LTM. Thus, transcriptional memory traces occur in different neurons within the same neural circuit.
巻・号 7
公開日 2018-10-22
DOI 10.7554/eLife.39196
PII 39196
PMID 30346271
PMC PMC6234028
MeSH Alleles Animals Appetite / physiology* Cyclic AMP Response Element-Binding Protein / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism* Gene Knockout Techniques Memory, Long-Term Mushroom Bodies / innervation* Mushroom Bodies / metabolism* Neurons / metabolism* Reproducibility of Results Trans-Activators / metabolism*
IF 7.551
引用数 5
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