論文 - 詳細
| RRC ID | 57195 |
|---|---|
| 著者 | Jha A, van Zanten TS, Philippe JM, Mayor S, Lecuit T. |
| タイトル | Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis. |
| ジャーナル | Curr Biol |
| Abstract |
Tissue morphogenesis arises from controlled cell deformations in response to cellular contractility. During Drosophila gastrulation, apical activation of the actomyosin networks drives apical constriction in the invaginating mesoderm and cell-cell intercalation in the extending ectoderm. Myosin II (MyoII) is activated by cell-surface G protein-coupled receptors (GPCRs), such as Smog and Mist, that activate G proteins, the small GTPase Rho1, and the kinase Rok. Quantitative control over GPCR and Rho1 activation underlies differences in deformation of mesoderm and ectoderm cells. We show that GPCR Smog activity is concentrated on two different apical plasma membrane compartments, i.e., the surface and plasma membrane invaginations. Using fluorescence correlation spectroscopy, we probe the surface of the plasma membrane, and we show that Smog homo-clusters in response to its activating ligand Fog. Endocytosis of Smog is regulated by the kinase Gprk2 and β-arrestin-2 that clears active Smog from the plasma membrane. When Fog concentration is high or endocytosis is low, Smog rearranges in homo-clusters and accumulates in plasma membrane invaginations that are hubs for Rho1 activation. Lastly, we find higher Smog homo-cluster concentration and numerous apical plasma membrane invaginations in the mesoderm compared to the ectoderm, indicative of reduced endocytosis. We identify that dynamic partitioning of active Smog at the surface of the plasma membrane or plasma membrane invaginations has a direct impact on Rho1 signaling. Plasma membrane invaginations accumulate high Rho1-guanosine triphosphate (GTP) suggesting they form signaling centers. Thus, Fog concentration and Smog endocytosis form coupled regulatory processes that regulate differential Rho1 and MyoII activation in the Drosophila embryo. |
| 巻・号 | 28(10) |
| ページ | 1570-1584.e6 |
| 公開日 | 2018-5-21 |
| DOI | 10.1016/j.cub.2018.03.068 |
| PII | S0960-9822(18)30426-3 |
| PMID | 29731302 |
| PMC | PMC6934411 |
| MeSH | Animals Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Endocytosis* Epithelium / growth & development Morphogenesis* Signal Transduction |
| IF | 9.193 |
| 引用数 | 7 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 35 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| ショウジョウバエ | |