| RRC ID |
572
|
| 著者 |
Yamamoto K, Zhang P, Banno Y, Fujii H, Miake F, Kashige N, Aso Y.
|
| タイトル |
Superoxide dismutase from the silkworm, Bombyx mori: sequence, distribution, and overexpression.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Superoxide dismutase (SOD) is an enzyme facilitating the removal of superoxide anions from living organisms. This study focused on SOD from the silkworm, Bombyx mori (bmSOD). cDNA encoding bmSOD was amplified by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of bmSOD indicated that the residues forming the Cu/Zn binding site are conserved and that the sequence is in 60% identity to that of the Drosophila melanogaster. B. mori SOD was also close to the D. melanogaster SOD in a phylogenetic tree. The bmSOD mRNA and the enzyme activity were widely distributed in diverse tissues. bmSOD functionally overexpressed in Escherichia coli in a soluble form was purified, and its stability was examined. bmSOD at 4 degrees C retained almost all of its original activity after incubation at pH 4-11 for 24 h. Incubation (pH 7) for 30 min at temperatures below 40 degrees C also affected activity insignificantly.
|
| 巻・号 |
69(3)
|
| ページ |
507-14
|
| 公開日 |
2005-3-1
|
| DOI |
10.1271/bbb.69.507
|
| PII |
JST.JSTAGE/bbb/69.507
|
| PMID |
15784978
|
| MeSH |
Amino Acid Sequence
Animals
Bombyx / enzymology*
Escherichia coli / genetics
Molecular Sequence Data
Phylogeny
RNA, Messenger / genetics
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Sequence Homology, Amino Acid
Superoxide Dismutase / chemistry
Superoxide Dismutase / genetics
Superoxide Dismutase / isolation & purification*
|
| IF |
1.516
|
| 引用数 |
21
|
|
WOS 分野
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
p50
EST database |
