| RRC ID |
57200
|
| Author |
Duan R, Kim JH, Shilagardi K, Schiffhauer ES, Lee DM, Son S, Li S, Thomas C, Luo T, Fletcher DA, Robinson DN, Chen EH.
|
| Title |
Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusion.
|
| Journal |
Nat Cell Biol
|
| Abstract |
Spectrin is a membrane skeletal protein best known for its structural role in maintaining cell shape and protecting cells from mechanical damage. Here, we report that α/βH-spectrin (βH is also called karst) dynamically accumulates and dissolves at the fusogenic synapse between fusing Drosophila muscle cells, where an attacking fusion partner invades its receiving partner with actin-propelled protrusions to promote cell fusion. Using genetics, cell biology, biophysics and mathematical modelling, we demonstrate that spectrin exhibits a mechanosensitive accumulation in response to shear deformation, which is highly elevated at the fusogenic synapse. The transiently accumulated spectrin network functions as a cellular fence to restrict the diffusion of cell-adhesion molecules and a cellular sieve to constrict the invasive protrusions, thereby increasing the mechanical tension of the fusogenic synapse to promote cell membrane fusion. Our study reveals a function of spectrin as a mechanoresponsive protein and has general implications for understanding spectrin function in dynamic cellular processes.
|
| Volume |
20(6)
|
| Pages |
688-698
|
| Published |
2018-6-1
|
| DOI |
10.1038/s41556-018-0106-3
|
| PII |
10.1038/s41556-018-0106-3
|
| PMID |
29802406
|
| PMC |
PMC6397639
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Fusion*
Cell Line
Cell Membrane / metabolism*
Cell Membrane / ultrastructure
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism*
Drosophila melanogaster / ultrastructure
Mechanotransduction, Cellular*
Membrane Fusion*
Mice
Microscopy, Fluorescence
Models, Biological
Myoblasts / metabolism*
Myoblasts / ultrastructure
Myoblasts, Skeletal / metabolism
Spectrin / genetics
Spectrin / metabolism*
Stress, Mechanical
Time Factors
|
| IF |
17.728
|
| Times Cited |
10
|
| Resource |
| Drosophila |
|
