RRC ID 57206
Author Kline A, Curry T, Lewellyn L.
Title The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
Journal Dev Biol
Abstract Intercellular bridges are conserved structures that allow neighboring cells to exchange cytoplasmic material; defects in intercellular bridges can lead to infertility in many organisms. Here, we use the Drosophila egg chamber to study the mechanisms that regulate intercellular bridges. Within the developing egg chamber, the germ cells (15 nurse cells and 1 oocyte) are connected to each other through intercellular bridges called ring canals, which expand over the course of oogenesis to support the transfer of materials from the nurse cells to the oocyte. The ring canals are enriched in actin and actin binding proteins, and many proteins have been identified that localize to the germline ring canals and control their expansion and stability. Here, we demonstrate a novel role for the Ste20 family kinase, Misshapen (Msn), in regulation of the size of the germline ring canals. Msn localizes to ring canals throughout most of oogenesis, and depletion of Msn led to the formation of larger ring canals. Over-expression of Msn decreased ring canal diameter, and expression of a membrane tethered form of Msn caused ring canal detachment and nurse cell fusion. Altering the levels or localization of Msn also led to changes in the actin cytoskeleton and altered the localization of E-cadherin, which suggests that Msn could be indirectly limiting ring canal size by altering the structure or dynamics of the actin cytoskeleton and/or adherens junctions.
Volume 440(2)
Pages 99-112
Published 2018-8-15
DOI 10.1016/j.ydbio.2018.05.006
PII S0012-1606(18)30067-8
PMID 29753016
PMC PMC6082181
MeSH Actin Cytoskeleton / metabolism Actins / metabolism Animals Contractile Proteins / metabolism Drosophila / genetics Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Female Germ Cells / cytology Germ Cells / metabolism Germ Cells / physiology* Microfilament Proteins / metabolism Oocytes / cytology Oocytes / enzymology Oogenesis / physiology Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism* Protein-Serine-Threonine Kinases / physiology*
IF 2.936
Times Cited 2
Resource
Drosophila