| RRC ID |
57213
|
| Author |
Huang YS, Ku HY, Tsai YC, Chang CH, Pao SH, Sun YH, Chiou A.
|
| Title |
5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila.
|
| Journal |
Sci Rep
|
| Abstract |
5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments.
|
| Volume |
7
|
| Pages |
44945
|
| Published |
2017-3-21
|
| DOI |
10.1038/srep44945
|
| PII |
srep44945
|
| PMID |
28322328
|
| PMC |
PMC5359570
|
| MeSH |
Animals
Biomarkers
Drosophila melanogaster / genetics*
Drosophila melanogaster / metabolism
Eye / metabolism*
Gene Expression
Gene Expression Regulation, Developmental
Genes, Reporter
Imaging, Three-Dimensional
Microscopy, Fluorescence / methods*
|
| IF |
4.011
|
| Times Cited |
4
|
| Resource |
| Drosophila |
|
