RRC ID 57215
Author Matsubayashi Y, Louani A, Dragu A, Sánchez-Sánchez BJ, Serna-Morales E, Yolland L, Gyoergy A, Vizcay G, Fleck RA, Heddleston JM, Chew TL, Siekhaus DE, Stramer BM.
Title A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation.
Journal Curr Biol
Abstract The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3-5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6-9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components.
Volume 27(22)
Pages 3526-3534.e4
Published 2017-11-20
DOI 10.1016/j.cub.2017.10.001
PII S0960-9822(17)31269-1
PMID 29129537
PMC PMC5714436
MeSH Animals Basement Membrane / metabolism Basement Membrane / physiology* Cell Movement / physiology Collagen / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / embryology Drosophila melanogaster / metabolism Epithelial Cells / metabolism Extracellular Matrix / metabolism* Extracellular Matrix / physiology Macrophages / metabolism
IF 9.193
Times Cited 26