論文 - 詳細
| RRC ID | 57218 |
|---|---|
| 著者 | Brock AR, Seto M, Smith-Bolton RK. |
| タイトル | Cap-n-Collar Promotes Tissue Regeneration by Regulating ROS and JNK Signaling in the Drosophila melanogaster Wing Imaginal Disc. |
| ジャーナル | Genetics |
| Abstract |
Regeneration is a complex process that requires an organism to recognize and repair tissue damage, as well as grow and pattern new tissue. Here, we describe a genetic screen to identify novel regulators of regeneration. We ablated the Drosophila melanogaster larval wing primordium by inducing apoptosis in a spatially and temporally controlled manner and allowed the tissue to regenerate and repattern. To identify genes that regulate regeneration, we carried out a dominant-modifier screen by assessing the amount and quality of regeneration in adult wings heterozygous for isogenic deficiencies. We have identified 31 regions on the right arm of the third chromosome that modify the regenerative response. Interestingly, we observed several distinct phenotypes: mutants that regenerated poorly, mutants that regenerated faster or better than wild-type, and mutants that regenerated imperfectly and had patterning defects. We mapped one deficiency region to cap-n-collar (cnc), the Drosophila Nrf2 ortholog, which is required for regeneration. Cnc regulates reactive oxygen species levels in the regenerating epithelium, and affects c-Jun N-terminal protein kinase (JNK) signaling, growth, debris localization, and pupariation timing. Here, we present the results of our screen and propose a model wherein Cnc regulates regeneration by maintaining an optimal level of reactive oxygen species to promote JNK signaling. |
| 巻・号 | 206(3) |
| ページ | 1505-1520 |
| 公開日 | 2017-7-1 |
| DOI | 10.1534/genetics.116.196832 |
| PII | genetics.116.196832 |
| PMID | 28512185 |
| PMC | PMC5500147 |
| MeSH | Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Imaginal Discs / metabolism* Imaginal Discs / physiology MAP Kinase Kinase 4 / metabolism MAP Kinase Signaling System Reactive Oxygen Species / metabolism* Regeneration* Repressor Proteins / genetics Repressor Proteins / metabolism* Wings, Animal / growth & development Wings, Animal / metabolism |
| IF | 3.564 |
| 引用数 | 14 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| ショウジョウバエ | |