RRC ID 57222
Author He L, Huang J, Perrimon N.
Title Development of an optimized synthetic Notch receptor as an in vivo cell-cell contact sensor.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Detection and manipulation of direct cell-cell contact in complex tissues is a fundamental and challenging problem in many biological studies. Here, we report an optimized Notch-based synthetic receptor (synNQ) useful to study direct cell-cell interactions in Drosophila With the synNQ system, cells expressing a synthetic receptor, which contains Notch activation machinery and a downstream transcriptional activator, QF, are activated by a synthetic GFP ligand expressed by contacting neighbor cells. To avoid cis-inhibition, mutually exclusive expression of the synthetic ligand and receptor is achieved using the "flippase-out" system. Expression of the synthetic GFP ligand is controlled by the Gal4/UAS system for easy and broad applications. Using synNQ, we successfully visualized cell-cell interactions within and between most fly tissues, revealing previously undocumented cell-cell contacts. Importantly, in addition to detection of cells in contact with one another, synNQ allows for genetic manipulation in all cells in contact with a targeted cell population, which we demonstrate in the context of cell competition in developing wing disks. Altogether, the synNQ genetic system will enable a broad range of studies of cell contact in developmental biology.
Volume 114(21)
Pages 5467-5472
Published 2017-5-23
DOI 10.1073/pnas.1703205114
PII 1703205114
PMID 28490499
PMC PMC5448223
MeSH Animals Caenorhabditis elegans Cell Line Drosophila Drosophila Proteins / chemical synthesis* Epithelial Cells / physiology Green Fluorescent Proteins Intercellular Junctions* Mice Receptors, Notch
IF 9.504