論文 - 詳細
| RRC ID | 57222 |
|---|---|
| 著者 | He L, Huang J, Perrimon N. |
| タイトル | Development of an optimized synthetic Notch receptor as an in vivo cell-cell contact sensor. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Detection and manipulation of direct cell-cell contact in complex tissues is a fundamental and challenging problem in many biological studies. Here, we report an optimized Notch-based synthetic receptor (synNQ) useful to study direct cell-cell interactions in Drosophila With the synNQ system, cells expressing a synthetic receptor, which contains Notch activation machinery and a downstream transcriptional activator, QF, are activated by a synthetic GFP ligand expressed by contacting neighbor cells. To avoid cis-inhibition, mutually exclusive expression of the synthetic ligand and receptor is achieved using the "flippase-out" system. Expression of the synthetic GFP ligand is controlled by the Gal4/UAS system for easy and broad applications. Using synNQ, we successfully visualized cell-cell interactions within and between most fly tissues, revealing previously undocumented cell-cell contacts. Importantly, in addition to detection of cells in contact with one another, synNQ allows for genetic manipulation in all cells in contact with a targeted cell population, which we demonstrate in the context of cell competition in developing wing disks. Altogether, the synNQ genetic system will enable a broad range of studies of cell contact in developmental biology. |
| 巻・号 | 114(21) |
| ページ | 5467-5472 |
| 公開日 | 2017-5-23 |
| DOI | 10.1073/pnas.1703205114 |
| PII | 1703205114 |
| PMID | 28490499 |
| PMC | PMC5448223 |
| MeSH | Animals Caenorhabditis elegans Cell Line Drosophila Drosophila Proteins / chemical synthesis* Epithelial Cells / physiology Green Fluorescent Proteins Intercellular Junctions* Mice Receptors, Notch |
| IF | 9.58 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |