RRC ID 57257
Author Hsu TH, Yang CY, Yeh TH, Huang YC, Wang TW, Yu JY.
Title The Hippo pathway acts downstream of the Hedgehog signaling to regulate follicle stem cell maintenance in the Drosophila ovary.
Journal Sci Rep
Abstract The Hippo pathway is conserved and plays important roles in organ size control. The core components of the Hippo pathway are two kinases Hippo (Hpo), Warts (Wts), and a transcription-co-activator Yorkie (Yki). Yki activity is regulated by phosphorylation, which affects its nuclear localization and stability. To determine the role of the Hippo pathway in stem cells, we examine follicle stem cells (FSCs) in the Drosophila ovary. Yki is detected in the nucleus of FSCs. Knockdown of yki in the follicle cell lineage leads to a disruption of the follicular epithelium. Mitotic clones of FSCs mutant for hpo or wts are maintained in the niche and tend to replace the other FSCs, and FSCs mutant for yki are rapidly lost, demonstrating that the Hippo pathway is both required and sufficient for FSC maintenance. Using genetic interaction analyses, we demonstrate that the Hedgehog pathway acts upstream of the Hippo pathway in regulating FSC maintenance. The nuclear localization of Yki is enhanced when the Hedgehog signaling is activated. Furthermore, a constitutively active but not a wild-type Yki promotes FSC maintenance as activation of the Hedgehog signaling does, suggesting that the Hedgehog pathway regulates Yki through a post-translational mechanism in maintaining FSCs.
Volume 7(1)
Pages 4480
Published 2017-6-30
DOI 10.1038/s41598-017-04052-6
PII 10.1038/s41598-017-04052-6
PMID 28667262
PMC PMC5493701
MeSH Animals Biomarkers Cell Differentiation / genetics Cell Lineage Cell Self Renewal Drosophila Drosophila Proteins / metabolism* Female Fluorescent Antibody Technique Hedgehog Proteins / metabolism* Intracellular Signaling Peptides and Proteins / metabolism* Nuclear Proteins / metabolism Ovarian Follicle / metabolism* Protein Binding Protein-Serine-Threonine Kinases / metabolism* RNA Processing, Post-Transcriptional Signal Transduction* Stem Cells / cytology Stem Cells / metabolism* Trans-Activators / metabolism
IF 4.011
Times Cited 6