論文 - 詳細
| RRC ID | 57299 |
|---|---|
| 著者 | Martin JL, Sanders EN, Moreno-Roman P, Jaramillo Koyama LA, Balachandra S, Du X, O'Brien LE. |
| タイトル | Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss. |
| ジャーナル | Elife |
| Abstract |
Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult Drosophila midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. This approach prolongs imaging sessions to 12-16 hr and yields movies that document cell and tissue dynamics at vivid spatiotemporal resolution. By applying a pipeline for movie processing and analysis, we uncover new and intriguing cell behaviors: that mitotic stem cells dynamically re-orient, that daughter cells use slow kinetics of Notch activation to reach a fate-specifying threshold, and that enterocytes extrude via ratcheted constriction of a junctional ring. By enabling real-time study of midgut phenomena that were previously inaccessible, our platform opens a new realm for dynamic understanding of adult organ renewal. |
| 巻・号 | 7 |
| 公開日 | 2018-11-14 |
| DOI | 10.7554/eLife.36248 |
| PII | 36248 |
| PMID | 30427308 |
| PMC | PMC6277200 |
| MeSH | Aging / physiology* Animals Cell Communication Cell Differentiation* Cell Division* Cell Lineage Cell Nucleus / metabolism Cell Survival Cell Tracking Digestive System / cytology* Drosophila melanogaster / cytology* Drosophila melanogaster / physiology* Enterocytes / cytology Imaging, Three-Dimensional Kinetics Mitosis Receptors, Notch / metabolism Single-Cell Analysis Stem Cells / cytology |
| IF | 7.551 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 89 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#112304 |