RRC ID 57302
Author Suzuki T, Takayama R, Sato M.
Title eyeless/Pax6 controls the production of glial cells in the visual center of Drosophila melanogaster.
Journal Dev Biol
Abstract Pax6 is known as a neurogenic factor in the development of the central nervous system and regulates proliferation of neuronal progenitor cells and promotes neuronal differentiation. In addition to neurogenesis, Pax6 is also involved in the specification and maturation of glial cells. Here, we show that Eyeless (Ey), Drosophila homolog of Pax6, regulates the production of glial cells in the brain. In the developing fly visual center, the production of neurons and glial cells are controlled by the temporal transcription factors that are sequentially expressed in neuroblasts (NBs). Among them, NBs of the last temporal window produce astrocyte-like glial cells. Ey is strongly expressed in the middle aged NBs, whose temporal window is earlier compared with glia producing older NBs. Weak Ey expression is also detected in the glia producing NBs. Our results suggest that Ey expression in the middle aged NBs indirectly control gliogenesis from the oldest NBs by regulating other temporal transcription factors. Additionally, weak Ey expression in the NBs of last temporal window may directly control gliogenesis. Ey is also expressed in neurons produced from the NBs of Ey-positive temporal window. Interestingly, neuron-specific overexpression of Ey causes significant increase in glial cells suggesting that neuronal expression of Ey may also contribute to gliogenesis. Thus, Pax6-dependent regulation of astrocyte-like glial development is conserved throughout the animal kingdom.
Volume 409(2)
Pages 343-53
Published 2016-1-15
DOI 10.1016/j.ydbio.2015.12.004
PII S0012-1606(15)30332-8
PMID 26670857
MeSH Animals Cell Proliferation DNA-Binding Proteins / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Models, Biological Neuroglia / cytology Neuroglia / metabolism* Neurons / cytology Neurons / metabolism Time Factors Visual Pathways / cytology* Visual Pathways / metabolism*
IF 2.936
Times Cited 7
Drosophila DGRC#104266