RRC ID 57309
Author Chang YC, Tu H, Chen JY, Chang CC, Yang SY, Pi H.
Title Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
Journal PLoS Genet
Abstract Stem cells rely on instructive cues from their environment. Alterations in microenvironments might contribute to tissue dysfunction and disease pathogenesis. Germline stem cells (GSCs) and cyst stem cells (CySC) in Drosophila testes are normally maintained in the apical area by the testicular hub. In this study, we found that reproduction leads to accumulation of early differentiating daughters of CySCs and GSCs in the testes of aged male flies, due to hyperactivation of Jun-N-terminal kinase (JNK) signaling to maintain self-renewal gene expression in the differentiating cyst cells. JNK activity is normally required to maintain CySCs in the apical niche. A muscle sheath surrounds the Drosophila testis to maintain its long coiled structure. Importantly, reproduction triggers accumulation of the tumor necrosis factor (TNF) Eiger in the testis muscle to activate JNK signaling via the TNF receptor Grindelwald in the cyst cells. Reducing Eiger activity in the testis muscle sheath suppressed reproduction-induced differentiation defects, but had little effect on testis homeostasis of unmated males. Our results reveal that reproduction in males provokes a dramatic shift in the testicular microenvironment, which impairs tissue homeostasis and spermatogenesis in the testes.
Volume 15(7)
Pages e1008062
Published 2019-7-1
DOI 10.1371/journal.pgen.1008062
PMID 31295251
PMC PMC6622487
MeSH Adult Germline Stem Cells / cytology* Adult Germline Stem Cells / metabolism Animals Cell Differentiation Cell Self Renewal Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / physiology* Female Homeostasis MAP Kinase Signaling System Male Membrane Proteins / metabolism Reproduction* Sexual Behavior, Animal Spermatogenesis Stem Cell Niche Testis / cytology Testis / metabolism
IF 5.224
Times Cited 2
Drosophila 9855R-1 5562R-3 4353R-2 15507R-2