RRC ID 57312
Author Goda S, Koga T, Yamashita K, Kuriura R, Ueda T.
Title A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii.
Journal Biosci. Biotechnol. Biochem.
Abstract In Archaea and Bacteria, surface layer (S-layer) proteins form the cell envelope and are involved in cell protection. In the present study, a putative S-layer protein was purified from the crude extract of Pyrococcus horikoshii using affinity chromatography. The S-layer gene was cloned and expressed in Escherichia coli. Isothermal titration calorimetry analyses showed that the S-layer protein bound N-acetylglucosamine and induced agglutination of the gram-positive bacterium Micrococcus lysodeikticus. The protein comprised a 21-mer structure, with a molecular mass of 1,340 kDa, as determined using small-angle X-ray scattering. This protein showed high thermal stability, with a midpoint of thermal denaturation of 79 °C in dynamic light scattering experiments. This is the first description of the carbohydrate-binding archaeal S-layer protein and its characteristics.
Volume 82(8)
Pages 1327-1334
Published 2018-8
DOI 10.1080/09168451.2018.1460571
PMID 29629656
MeSH Acetylglucosamine / metabolism* Amino Acid Sequence Archaeal Proteins / chemistry Archaeal Proteins / genetics Archaeal Proteins / isolation & purification Archaeal Proteins / metabolism* Calorimetry / methods Chromatography, Affinity / methods Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli / genetics Genes, Archaeal Green Fluorescent Proteins / metabolism Hot Temperature Micrococcus / metabolism Protein Binding Protein Conformation Protein Denaturation Protein Stability Pyrococcus horikoshii / metabolism* Scattering, Small Angle X-Ray Diffraction
IF 1.255
Resource
General Microbes JCM 9975