Reference - Detail
| RRC ID | 57314 |
|---|---|
| Author | Sugiyama Y, Nara M, Sakanaka M, Kitakata A, Okuda S, Kurihara S. |
| Title | Analysis of polyamine biosynthetic- and transport ability of human indigenous Bifidobacterium. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Bifidobacteria are members of the human intestinal microbiota, being numerically dominant in the colon of infants, and also being prevalent in the large intestine of adults. In this study, we measured the concentrations of major polyamines (putrescine, spermidine, and spermine) in cells and culture supernatant of 13 species of human indigenous Bifidobacterium at growing and stationary phase. Except for Bifidobacterium bifidum and Bifidobacterium gallicum, 11 species contained spermidine and/or spermine when grown in Gifu-anaerobic medium (GAM). However, Bifidobacterium scardovii and Bifidobacterium longum subsp. infantis, which contain spermidine when grown in GAM, did not contain spermidine when grown in polyamine-free 199 medium. Of the tested 13 Bifidobacterium species, 10 species showed polyamine transport ability. Combining polyamine concentration analysis in culture supernatant and in cells, with basic local alignment search tool analysis suggested that novel polyamine transporters are present in human indigenous Bifidobacterium. ABBREVIATIONS:Put: putrescine; Spd: spermidine; Spm: spermine; GAM: Gifu anaerobic medium; BHI: brain-heart infusion. |
| Volume | 82(9) |
| Pages | 1606-1614 |
| Published | 2018-9-1 |
| DOI | 10.1080/09168451.2018.1475211 |
| PMID | 29847302 |
| MeSH | Anaerobiosis Bifidobacterium / classification Bifidobacterium / metabolism* Biological Transport Chromatography, High Pressure Liquid Culture Media Humans Membrane Transport Proteins / metabolism Putrescine / biosynthesis* Species Specificity Spermidine / biosynthesis* Spermine / biosynthesis* |
| IF | 1.297 |
| Times Cited | 3 |
| Resource | |
| General Microbes | JCM 8224, JCM 15439, JCM 1222, JCM 1217, JCM 1200, JCM 12489, JCM 1275, JCM 7096, JCM 10602, JCM 1254, JCM 1192, JCM 1194, JCM 19861 |