| RRC ID |
5731
|
| Author |
Nunoura T, Oida H, Miyazaki J, Miyashita A, Imachi H, Takai K.
|
| Title |
Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities.
|
| Journal |
FEMS Microbiol Ecol
|
| Abstract |
A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set for the methyl coenzyme M reductase subunit A gene (mcrA). The method developed was applied to several microbial communities in which diversity and abundance of methanogens or anaerobic methanotrophs (ANMEs) was identified by 16S rRNA gene clone analysis, and strong correlations between the copy numbers of mcrA with those of archaeal 16S rRNA genes in the communities were observed. The assay can be applied to detecting and assessing the abundance of methanogens and/or ANMEs in anoxic environments that could not be detected by 16S rRNA gene sequence analyses.
|
| Volume |
64(2)
|
| Pages |
240-7
|
| Published |
2008-5-1
|
| DOI |
10.1111/j.1574-6941.2008.00451.x
|
| PII |
FEM451
|
| PMID |
18318714
|
| MeSH |
Archaea / enzymology*
Archaeal Proteins / genetics
DNA Primers / genetics
DNA, Archaeal / genetics
DNA, Ribosomal / genetics
Fluorescence
Gene Dosage*
Methane / metabolism*
Oxidoreductases / genetics*
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
|
| IF |
3.675
|
| Times Cited |
69
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM10825
JCM13029
JCM10723
JCM10045
JCM10549
JCM11834
JCM9639 |
