RRC ID 57322
著者 Koike T, Harada K, Horiuchi S, Kitamura D.
タイトル The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.
ジャーナル Elife
Abstract In mice, memory B (Bmem) cells can be divided into two subpopulations: CD80hi Bmem cells, which preferentially differentiate into plasma cells; and CD80lo Bmem cells, which become germinal center (GC) B cells during a recall response. We demonstrate that these distinct responses can be B-cell-intrinsic and essentially independent of B-cell receptor (BCR) isotypes. Furthermore, we find that the development of CD80hi Bmem cells in the primary immune response requires follicular helper T cells, a relatively strong CD40 signal and a high-affinity BCR on B cells, whereas the development of CD80lo Bmem cells does not. Quantitative differences in CD40 stimulation were enough to recapitulate the distinct B cell fate decisions in an in vitro culture system. The quantity of CD40 signaling appears to be translated into NF-κB activation, followed by BATF upregulation that promotes Bmem cell differentiation from GC B cells.
巻・号 8
公開日 2019-6-21
DOI 10.7554/eLife.44245
PII 44245
PMID 31225793
PMC PMC6636905
MeSH Animals B-Lymphocytes / immunology* B7-1 Antigen / genetics CD40 Antigens / genetics CD40 Antigens / immunology* Cell Differentiation / genetics Cell Lineage / genetics Cell Lineage / immunology Germinal Center / immunology Immunoglobulin Isotypes Immunologic Memory / genetics* Immunologic Memory / immunology Mice NF-kappa B / genetics NF-kappa B / immunology Plasma Cells / immunology Receptors, Antigen, B-Cell / genetics* Receptors, Antigen, B-Cell / immunology Signal Transduction T-Lymphocytes, Helper-Inducer / immunology
IF 7.551
引用数 3
リソース情報
実験動物マウス RBRC05663
ヒト・動物細胞 BALB/3T3 clone A31(RCB0005)