論文 - 詳細
RRC ID | 57322 |
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著者 | Koike T, Harada K, Horiuchi S, Kitamura D. |
タイトル | The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. |
ジャーナル | Elife |
Abstract |
In mice, memory B (Bmem) cells can be divided into two subpopulations: CD80hi Bmem cells, which preferentially differentiate into plasma cells; and CD80lo Bmem cells, which become germinal center (GC) B cells during a recall response. We demonstrate that these distinct responses can be B-cell-intrinsic and essentially independent of B-cell receptor (BCR) isotypes. Furthermore, we find that the development of CD80hi Bmem cells in the primary immune response requires follicular helper T cells, a relatively strong CD40 signal and a high-affinity BCR on B cells, whereas the development of CD80lo Bmem cells does not. Quantitative differences in CD40 stimulation were enough to recapitulate the distinct B cell fate decisions in an in vitro culture system. The quantity of CD40 signaling appears to be translated into NF-κB activation, followed by BATF upregulation that promotes Bmem cell differentiation from GC B cells. |
巻・号 | 8 |
公開日 | 2019-6-21 |
DOI | 10.7554/eLife.44245 |
PII | 44245 |
PMID | 31225793 |
PMC | PMC6636905 |
MeSH | Animals B-Lymphocytes / immunology* B7-1 Antigen / genetics CD40 Antigens / genetics CD40 Antigens / immunology* Cell Differentiation / genetics Cell Lineage / genetics Cell Lineage / immunology Germinal Center / immunology Immunoglobulin Isotypes Immunologic Memory / genetics* Immunologic Memory / immunology Mice NF-kappa B / genetics NF-kappa B / immunology Plasma Cells / immunology Receptors, Antigen, B-Cell / genetics* Receptors, Antigen, B-Cell / immunology Signal Transduction T-Lymphocytes, Helper-Inducer / immunology |
IF | 7.551 |
引用数 | 3 |
リソース情報 | |
実験動物マウス | RBRC05663 |
ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |