RRC ID 57369
Author Tokusumi Y, Tokusumi T, Schulz RA.
Title Mechanical stress to Drosophila larvae stimulates a cellular immune response through the JAK/STAT signaling pathway.
Journal Biochem Biophys Res Commun
Abstract Acute inflammation can cause serious tissue damage and disease in physiologically-challenged organisms. The precise mechanisms leading to these detrimental effects remain to be determined. In this study, we utilize a reproducible means to induce cellular immune activity in Drosophila larvae in response to mechanical stress. That is, forceps squeeze-administered stress induces lamellocytes, a defensive hemocyte type that normally appears in response to wasp infestation of larvae. The posterior signaling center (PSC) is a cellular microenvironment in the larval hematopoietic lymph gland that is vital for lamellocyte induction upon parasitoid attack. However, we found the PSC was not required for mechanical stress-induced lamellocyte production. In addition, we observed that mechanical injury caused a systemic expression of Unpaired3. This cytokine is both necessary and sufficient to activate the cellular immune response to the imposed stress. These findings provide new insights into the communication between injured tissues and immune system induction, using stress-challenged Drosophila larvae as a tractable model system.
Volume 502(3)
Pages 415-421
Published 2018-7-20
DOI 10.1016/j.bbrc.2018.05.192
PII S0006-291X(18)31281-6
PMID 29856996
MeSH Animals Animals, Genetically Modified Cellular Microenvironment Drosophila Proteins / metabolism Drosophila melanogaster / immunology* Drosophila melanogaster / metabolism* Drosophila melanogaster / parasitology Hemocytes / cytology Hemocytes / immunology Immunity, Cellular Janus Kinases / metabolism Larva / immunology Larva / metabolism Larva / parasitology STAT Transcription Factors / metabolism Signal Transduction Stress, Mechanical Transcription Factors / metabolism Wasps / immunology Wasps / pathogenicity
IF 2.705
Times Cited 5