RRC ID 57372
著者 Jagannathan M, Cummings R, Yamashita YM.
タイトル A conserved function for pericentromeric satellite DNA.
ジャーナル Elife
Abstract A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi-AT-hook satellite DNA-binding proteins, Drosophila melanogaster D1 and mouse HMGA1, play an evolutionarily conserved role in bundling pericentromeric satellite DNA from heterologous chromosomes into 'chromocenters', a cytological association of pericentromeric heterochromatin. Defective chromocenter formation leads to micronuclei formation due to budding from the interphase nucleus, DNA damage and cell death. We propose that chromocenter and satellite DNA serve a fundamental role in encapsulating the full complement of the genome within a single nucleus, the universal characteristic of eukaryotic cells.
巻・号 7
公開日 2018-3-26
DOI 10.7554/eLife.34122
PII 34122
PMID 29578410
PMC PMC5957525
MeSH Animals Cell Nucleus / metabolism* Chromosomes / metabolism* DNA, Satellite / metabolism* DNA-Binding Proteins / metabolism Drosophila Proteins / metabolism Drosophila melanogaster Eukaryotic Cells / physiology* HMGA1a Protein / metabolism Mice
IF 7.551
引用数 16
リソース情報
ショウジョウバエ DGRC#110806 DGRC#140754