論文 - 詳細
| RRC ID | 5738 |
|---|---|
| 著者 | Uzura A, Nomoto F, Sakoda A, Nishimoto Y, Kataoka M, Shimizu S. |
| タイトル | Stereoselective synthesis of (R)-3-quinuclidinol through asymmetric reduction of 3-quinuclidinone with 3-quinuclidinone reductase of Rhodotorula rubra. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
A novel nicotinamide adenine dinucleotide phosphate-dependent carbonyl reductase, 3-quinuclidinone reductase, was isolated from Rhodotorula rubra JCM3782. The enzyme catalyzes the asymmetric reduction of 3-quinuclidinone to (R)-3-quinuclidinol. The gene encoding the enzyme was also cloned and sequenced. A 819-bp nucleotide fragment was confirmed to be the gene encoding the 3-quinuclidinone reductase by agreement of the internal amino acid sequences of the purified enzyme. The gene encodes a total of 272 amino acid residues, and the deduced amino acid sequence shows similarity to those of several short-chain dehydrogenase/reductase family proteins. An expression vector, pWKLQ, which contains the full length 3-quinuclidinone reductase gene was constructed. Using Escherichia coli cells coexpressing the 3-quinuclidinone reductase and glucose dehydrogenase (cofactor regeneration enzyme) genes, 618 mM 3-quinuclidinone was almost stiochiometrically converted to (R)-3-quinuclidinol with an >99.9% enantiomeric excess within 21 h of reaction. |
| 巻・号 | 83(4) |
| ページ | 617-26 |
| 公開日 | 2009-6-1 |
| DOI | 10.1007/s00253-009-1902-2 |
| PMID | 19234697 |
| MeSH | Alcohol Oxidoreductases / genetics Alcohol Oxidoreductases / isolation & purification Alcohol Oxidoreductases / metabolism* Amino Acid Sequence Base Sequence Biotransformation Cloning, Molecular DNA, Fungal / chemistry DNA, Fungal / genetics Escherichia coli / genetics Fungal Proteins / genetics Fungal Proteins / isolation & purification Fungal Proteins / metabolism* Gene Expression Glucose 1-Dehydrogenase / genetics Glucose 1-Dehydrogenase / metabolism Molecular Sequence Data Quinuclidines / metabolism* Rhodotorula / enzymology* Rhodotorula / genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Stereoisomerism |
| IF | 3.53 |
| 引用数 | 26 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM3782 |