RRC ID |
5740
|
著者 |
Ohbuchi T, Takahashi T, Azumi N, Sakaino M.
|
タイトル |
Structual analysis of neutral and acidic xylooligosaccharides from hardwood kraft pulp, and their utilization by intestinal bacteria in vitro.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
We established a method for producing two types of xylooligosaccharides by treating kraft pulp derived from hardwood such as eucalyptus with xylanase, analyzed the structure by MALDI-TOF-MS/MS, and conducted an in vitro study on utilization by intestinal bacteria. One type was a neutral xylooligosaccharide (XOS), a xylooligomer in the D.P. range of dimer to approximately 17-mer, containing approximately 12% xylobiose. The other was an acidic xylooligosaccharide (U-XOS), a xylooligomer in the D.P. range of dimer to approximately 17-mer, with a major side chain of 4-O-methyl-glucuronic acid and other side chains of glucuronic acid and 2-O-galactopyranosyl-4-O-methyl-glucurono acid. The in vitro utilization study with intestinal bacteria showed that XOS was specifically utilized by Bifidobacterium, whereas U-XOS was utilized by only a few types of bacteria. In a fecal batch culture, both XOS and U-XOS selectively increased the population of Bifidobacterium. The present study proposes a new method for industrial production of XOS and U-XOS, and indicates the utility and functionality.
|
巻・号 |
73(9)
|
ページ |
2070-6
|
公開日 |
2009-9-1
|
DOI |
10.1271/bbb.90260
|
PII |
JST.JSTAGE/bbb/90260
|
PMID |
19734663
|
MeSH |
Bifidobacterium / metabolism*
Carbohydrate Conformation
Intestines / microbiology*
Oligosaccharides / chemistry*
Oligosaccharides / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Textile Industry*
Wood*
|
IF |
1.516
|
引用数 |
22
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
一般微生物 |
JCM1275
JCM1194
JCM1195
JCM1200
JCM7096
JCM1255
JCM1192
JCM1222
JCM1217
JCM1190
JCM1023
JCM1173
JCM1131
JCM1150
JCM5824
JCM1296
JCM1298
JCM1231 |