Reference - Detail
| RRC ID | 574 |
|---|---|
| Author | Yamamoto K, Zhang P, Miake F, Kashige N, Aso Y, Banno Y, Fujii H. |
| Title | Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori. |
| Journal | Comp Biochem Physiol B Biochem Mol Biol |
| Abstract |
This study focused on glutathione S-transferase (GST), one of the detoxification enzymes, from the silkworm, Bombyx mori (GSTT1). A cDNA encoding a putative GST was amplified by reverse transcriptase-polymerase chain reaction and sequenced. The deduced amino acid sequence revealed 59%, 57% and 56% identities to theta-class GSTs of Musca domestica, Anopheles gambiae and Drosophila melanogaster, respectively. GSTT1 was also estimated to be close to those GSTs in a phylogenetic tree. Recombinant GST (rGSTT1) was functionally overexpressed in Escherichia coli in a soluble form, purified to homogeneity, and characterized. The pH-optimum of rGSTT1 was broad from pH 4 to 9 and rGSTT1 retained more than 75% of its original activity after incubation at pH 5-11. Incubation for 30 min at temperatures below 50 degrees C also affected the activity insignificantly. The Michaelis constant for 1-chloro-2,4-dinitrobenzene was 0.48 mM. |
| Volume | 141(3) |
| Pages | 340-6 |
| Published | 2005-7-1 |
| DOI | 10.1016/j.cbpc.2005.04.012 |
| PII | S1096-4959(05)00084-9 |
| PMID | 15950511 |
| MeSH | Amino Acid Sequence Animals Bombyx / enzymology* Cloning, Molecular DNA, Complementary / genetics DNA, Complementary / metabolism Glutathione Transferase / genetics Glutathione Transferase / metabolism* Hydrogen-Ion Concentration Molecular Sequence Data Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism* Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature |
| IF | 2.219 |
| Times Cited | 64 |
| WOS Category | ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | p50 EST database |